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KMID : 0545120020120010172
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 1 p.172 ~ p.175
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Molecular Cloning of the nahC Gene Encoding 1,2-Dihydroxynaphthalene Dioxygenase from Pseudomonas fluorescens
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KIM, YEO-JUNG
LEE, NA-RI/CHOI, SOON-YOUNG/MIN, KYUNG-HEE
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Abstract
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The complete nucleotide sequence of the nahC gene from Pseudomonas fluorescens, the structural gene for 1,2-dihydroxynaphthalene (1,2-DHN) dioxygenase, was determined. The 1,2-DHN dioxygenase is an extradiol ring-cleavage enzyme that cleaves the first ring of 1,2-dihydroxynaphthalene. The amino acid sequence of the dioxygenase deduced from the nucleotide sequence suggested that the holoenzyme consists of eight identical subunits with a molecular weight of approximately 34,200. The amino acid sequence of 1,2-DHN dioxygenase showed more than 90% homology with those of the dioxygenases of other Pseudomonas strains. However, sequence similarity with those of the Sphingomonas species was less than 60%. The nahC gene of P. fluorescens was moderately expressed in E. coli NM522, as determined by enzymatic activity.
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